KAMRC ENTOMOLOGY

Entomology lab is established with main focus towards the study on Kala-azar Vector Phlebotomus argentipes (sand fly), its Epidemiology, Transmission Dynamics, Blood Meal Identification, Finding of Reservoir Host, Assess Insecticide Resistance etc. Entomology lab is well equipped with the instruments required in the entomological studies, and has a well-established closed colony of P. argentipes, developed in 2014 and certified as pathogen free. It also has a very good animal facility to facilitate blood feeding of sand flies for colony maintenance.
 
Existing infrastructure
 
Insect Dissection and Stereozoom Microscope
Phase Contrast Microscope
Environmental Chambers
Minus20, Minus 80 Deep Freeze
PCR
Electrophoresis
NanoDrop
Laminar Hood
BOD Incubator
Microcentrifuge
Membrane Feeder
Animal facility

 

Current Research Interests
Transmission dynamics of Visceral Leishmaniasis (VL)
Xenodiagnosis 
Finding of non-human reservoir host for VL.
Evaluation of susceptibility status and molecular basis of insecticide resistance of P. argentipes
 
 
Publications
 
1.      Om Prakash Singh, Puja Tiwary, Anurag Kumar Kushwaha, Shakti Kumar Singh, Dhiraj Kumar Singh, Phillip Lawyer, Edgar Rowton, Rahul Chaubey, Abhishek Kumar Singh, Tulika Kumari Rai, Michael P Fay, Jaya Chakravarty, David Sacks, Shyam Sundar.  Xenodiagnosis to evaluate the infectiousness of humans to sandflies in an area endemic for visceral leishmaniasis in Bihar, India: a transmission-dynamics study. The Lancet Microbe 2021; 2: e23–31.
 
2.      Puja Tiwary, Shakti Kumar Singh, Anurag Kumar Kushwaha, Edgar Rowton, David Sacks, Om Prakash Singh, Shyam Sundar, and Phillip Lawyer. Establishing, Expanding, and Certifying a Closed Colony of Phlebotomus argentipes (Diptera: Psychodidae) for Xenodiagnostic Studies at the Kala Azar Medical Research Center, Muzaffarpur, Bihar, India. Journal of Medical Entomology, 2017, 1–11; doi: 10.1093/jme/tjx099.
 
3.      Puja Tiwary, Dinesh Kumar, Mukesh Mishra, Rudra Pratap Singh, Madhukar Rai, Shyam Sundar. Seasonal Variation in the Prevalence of Sand Flies Infected with Leishmania donovani. PLoS ONE, 2013, 8(4): e61370. doi:10.1371/journal.pone.0061370.
 
4.      Puja TiwaryDinesh KumarMadhukar RaiShyam Sundar. PCR-RFLP Based Method for Molecular Differentiation of Sand Fly Species Phlebotomus argentipes, Phlebotomus papatasi, and Sergentomyia babu Found in India. Journal of Medical Entomology, 2012, 49 (6)1515-1518, https://doi.org/10.1603/ME12033.
 
5.      Puja Tiwary, Dinesh Kumar, Rudra Pratap Singh, Madhukar Rai, and Shyam Sundar. Prevalence of Sand Flies and Leishmania donovani Infection in a Natural Population of Female Phlebotomus argentipes in Bihar State, India. VECTOR-BORNE AND ZOONOTIC DISEASES, 2012, 12 (6). DOI: 10.1089/vbz.2011.0808.